106 samples from 4 sources were collected in 5 European regions and 30 faecal indicators and source tracking markers were evaluated
, including E. coli
, enterococci, clostridia, bifidobacteria, somatic coliphages, host-specific bacteria, human viruses, host mitochondrial DNA, host-specific bacteriophages and artificial sweeteners. Models based on linear discriminant analysis (LDA) able to distinguish between human and non-human faecal pollution and identify faecal pollution of several origins were developed and tested with 36 additional laboratory-made samples. With 5 variables the model correctly classified all the fresh faecal samples from 4 different sources.